Why Is Pcr Used In The Process Of Dna Sequencing Quizlet
Olivia Luz
Why is pcr used in the process of dna sequencing during sequencing fluorescent markers should be incorporated into the dna strand for the determination of the nucleotide sequence.
Sometimes called molecular photocopying the polymerase chain reaction pcr is a fast and inexpensive technique used to amplify copy small segments of dna. Which of these sequences is the complementary sequence to the template sequence 5 gtggtctgatcaacggtaa 3 3 caccagactagttgccatt 5 the number of repeats of each individual s tandem repeated regions can be different creating a specific dna profile. Generally the four types of dntps are incorporated into the newly synthesizing dna strand during pcr. The pcr is a process employed to amplify the dna and used in the dna sequencing as well to get dna copies to reduce contamination identify dna mutations and recombinant clones in the conventional pcr method in order to get copies of dna our gene of interest or dna is amplified enzymatically using the forward and reverse primer sets.
Creates ideal temperature where the certain enzyme and primers can work to copy. This is the ability to isolate strands from viruses and compare them to databases if desired. This incorporation takes place during pcr. Because significant amounts of a sample of dna are necessary for molecular and genetic analyses studies of isolated pieces of dna are nearly impossible without pcr amplification.
2 how can dna sequencing be used to identify other classes of pathogens such as viruses.
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